Adult dorsal root ganglia neurons are among the few adult neuronal cell types that can be purified Sport Optics and grown relatively easily in dissociated cell culture.Here we describe a procedure for the isolation and Wiring Harness growth of dissociated adult mouse DRG neurons using Percoll gradients and a chemically defined medium.These cultures can be used for morphological, biochemical and electrophysiological studies of neuronal growth and function.